ExperimentMic multiplexed FISH on IMR-90

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multiplexed FISH4DNEXCWE7KU7
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DNA-MERFISH combined with RNA MERFISH to detect nascent RNA transcripts of 1137 genes overlapping with 1,041 genomic loci targeted by DNA-MERFISH, Biological replicate 1, Technical replicate 1

   October 17th, 2024 at 2:36pm
Source Publication
Genome-Scale Imaging of the 3D Organization and Transcriptional Activity of Chromatin.
Su JH, Zheng P, et al., Cell 2020

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSUH1CEQ3
Biosample Biosource 
IMR-90 - 4DNSRXXGU3CH
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
-
Chromosomes targeted by DAPI
ch01
1-18
RNA-MERFISH to detect 1137 genes overlapping 1041 genomic loci (targeted by DNA MERFISH) by Alexa Flour 750-labeled imaging oligos
ch02
1-18
RNA-MERFISH to detect 1137 genes overlapping 1041 genomic loci (targeted by DNA MERFISH) by Alexa Flour 647-labeled imaging oligos
ch03
1-18
RNA-MERFISH to detect 1137 genes overlapping 1041 genomic loci (targeted by DNA MERFISH) by Cy3-labeled imaging oligos
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