ExperimentMic multiplexed FISH on multicellular organism

multiplexed FISH4DNEXBODJ1L2
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Multiplexed FISH of 3 TADs (TAD-12 to TAD-14) spanning the central region of chromosome V in wild-type (Bristol N2 strain) C. elegans, combined from at least 2 biological replicates.

   April 8th, 2025 at 4:16pm
Source Publication
Lamina-Dependent Stretching and Unconventional Chromosome Compartments in Early C. elegans Embryos.
Sawh AN, Shafer MER, et al., Molecular cell 2020

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBS5G94GPO
Biosample Biosource 
multicellular organism - 4DNSR6C4W4UN
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
-
DAPI
ch01
-
fiducial beads
ch02
1-20
3 TADs on ChrV (central region) imaged using ATTO 561 imaging probes
ch03
1-20
3 TADs on ChrV (central region) imaged using Alexa Fluor 647 imaging probes

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