ExperimentMic multiplexed FISH on A549

multiplexed FISH4DNEXB98WM6D
released

Multiplexed FISH of chromosome 22 in A549-Cas9 cancer cells from 17 coverslips, where each coverslip is imaging of the pooled CRISPR screen cells where cells are transduced with sgRNA targeting and knockdown specific genes or sgNTC, non-targeting control. The Crispr screen includes a total of 137 gene targeted by 404 sgRNAs and 8 sgNTC used as controls. The knocked out gene and NTC corresponding to a trace can be found in the associated fof-ct processed files., Biological Replicate 1, Technical Replicate 1

   March 28th, 2025 at 1:37pm
Source Publication
Perturb-tracing enables high-content screening of multiscale 3D genome regulators.
Cheng Y, Hu M, et al., bioRxiv : the preprint server for biology 2023

Properties  


Experiment Type 
Follows SOP 
No
Biosample 
Biosample Biosource 
Biosample Modifications 
Viral Transduction and Viral Transduction
Biosample Treatments 
None
Microscopy Technique 
None
Microscope Quality Control 
None
Microscope Configuration 
None

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