ExperimentMic multiplexed FISH on H9

multiplexed FISH4DNEX9M1MGSI
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Chromatin tracing of SOX9 locus in wild type H9 hESCs. 80 sequential steps spaced 50 kb (each spot is 5 kb), 4 Mb in total. Imaged together with the differentiated CNCCs condition. Replicate 1 of 1.

   January 18th, 2023 at 9:24pm
Source Publication
Structural elements promote architectural stripe formation and facilitate ultra-long-range gene regulation at a human disease locus.
Chen LF, Long HK, et al., Molecular cell 2023

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSHRCYJ8K
Biosample Biosource 
H9 - 4DNSRBJKA2H5
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Highly Inclined Laminated Optical Sheet
Microscope Quality Control 
None
Microscope Configuration 
Stanford-Boettiger_lab_ORCA_Nikon_Eclipse_Ti-U_Scope1
Channel
Imaging Rounds
Path
 
ch00
1-80
80 spaced regions at SOX9 locus targeted by oligo probe library, readout oligo library (with Cy5-labeled imaging oligos)
ch01
-
80 spaced regions at SOX9 locus targeted by oligo probe library (with Cy3-labeled fiducial oligo probes)

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