ExperimentMic multiplexed FISH on RPE-hTERT (Tier 2)

multiplexed FISH4DNEX75SP2JS
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Multiplexed FISH of chromosome 22 in RPE-1 cells from 2 coverslips where cells expressing non targeting siRNA, used as control for siCHD7-chr22, Biological Replicate 1, Technical Replicate 1

   March 28th, 2025 at 1:38pm
Source Publication
Perturb-tracing enables high-content screening of multiscale 3D genome regulators.
Cheng Y, Hu M, et al., bioRxiv : the preprint server for biology 2023

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSIFUC2B9
Biosample Biosource 
RPE-hTERT (Tier 2) - 4DNSRHGVFSRJ
Biosample Modifications 
Viral Transduction deletion for CHD7 gene
Biosample Treatments 
None
Microscopy Technique 
None
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
0
Chromosomes targeted by DAPI
ch01
1-14
Yellow-green fiducial beads for drift correction on chromosome 22
ch02
0
G1 phase detection
ch03
1-14
27 TADs imaged on Chr22 imaged using ATTO 565 imaging probes
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