ExperimentMic multiplexed FISH on 293T

multiplexed FISH4DNEX6Z2ATZQ
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Multiplexed FISH in HEK293T reporter cell line treated with doxycyline for 5 days resulting in accumulation of HDAC4 causing silencing of reporter gene expression in cells, Biological Replicate 3 ,Technical Replicate 1 (control and experiment relationships are described in detailed in 4DNFIWQ9QBOB supplementary file).

   February 4th, 2025 at 9:45pm
Source Publication
Single-cell chromatin state transitions during epigenetic memory formation.
Fujimori T, Rios-Martinez C, et al., bioRxiv : the preprint server for biology 2023

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSFECWS7I
Biosample Biosource 
293T - 4DNSRXG828T6
Biosample Modifications 
Viral transduction insertion of reporter construct integrated into AAVS1 safe harbor site on chr19 and Stable transfection for expression of rTetR-HDAC4
Biosample Treatments 
doxycycline treatment (1.0 μg/mL, 5.0d)
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
-
DAPI staining
ch01
1-19
Custom genome assembly (mCitrine reporter + 9 TetO sites inserted into AAVS1 safe harbor site 55115766 on chr19) targeted by Cy5-labeled imaging oligos
ch02
1-19
Custom genome assembly (mCitrine reporter + 9 TetO sites inserted into AAVS1 safe harbor site 55115766 on chr19) targeted by Cy3-labeled fiducial oligos

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