ExperimentMic multiplexed FISH on TC1 with EED-dTAG, dTAG-Ring1b

multiplexed FISH4DNEX6COY1F2
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Replicate 2 of Chromatin tracing (ORCA) of HoxA locus in TC1 mouse ESCs, with Pc factors EED and RING1b endogenously tagged with the dTAG inducible degrons, treated with dTAG-13 for 8 hours

   January 24th, 2023 at 12:49pm
Source Publication
Polycomb repression of Hox genes involves spatial feedback but not domain compaction or phase transition.
Murphy SE, Boettiger AN, Nature genetics 2024

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBST113LPL
Biosample Biosource 
TC1 with EED-dTAG, dTAG-Ring1b - 4DNSRTJHLMQ8
Biosample Modifications 
None
Biosample Treatments 
dTAG-13 treatment (500.0 nM, 8.0h)
Microscopy Technique 
Highly Inclined Laminated Optical Sheet
Microscope Quality Control 
None
Microscope Configuration 
Stanford-Boettiger_lab_ORCA_Nikon_Eclipse_Ti2-U_Scope3
Channel
Imaging Rounds
Path
 
ch00
1-52
52 consecutive 5-kb regions at HoxA locus (mouse) targeted by oligo probe library, readout oligo library (with Cy5-labeled imaging oligos)
ch01
1-52
52 consecutive 5-kb regions at HoxA locus (mouse) targeted by oligo probe library (with Cy3-labeled fiducial oligo probes)

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