ExperimentMic multiplexed FISH on LUAD from mouse SA3440 and LUAD cells from mouse MM1433 and LUAD cells from mouse MM2030 and LUAD cells from mouse SA3501 and LUAD cells from mouse MM1690

multiplexed FISH4DNEX4F82R7B
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Multiplexed FISH in GFP+ expressing (green) homozygous mutant (Trp53 -/-) LUAD cells from K-MADM-Trp53 mouse, Biological Relicate 1, Technical Replicate 1

   May 27th, 2025 at 5:41pm
Source Publication
Tracing the evolution of single-cell cancer 3D genomes: an atlas for cancer gene discovery.
Liu M, Jin S, et al., bioRxiv : the preprint server for biology 2024

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSGYLYO7P
Biosample Biosource 
  1. LUAD from mouse SA3440 - 4DNSRD4JLYQD
  2. LUAD cells from mouse MM1433 - 4DNSRBAWEU4R
  3. LUAD cells from mouse MM2030 - 4DNSR7TX4JLS
  4. LUAD cells from mouse SA3501 - 4DNSRQ63ELJQ
  5. LUAD cells from mouse MM1690 - 4DNSRV8GSS44
Biosample Modifications 
Viral Transduction
Biosample Treatments 
None
Microscopy Technique 
None
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
0
Yellow-green fiducial beads for drift correction
ch01
0
Chromosomes targeted by DAPI
ch02
1-50
~60kb feature-containing or central region of each of 473 TADs spanning from Chr1 to Ch19 targeted by Alexa 647-labelled imaging oligos
ch03
1-50
~60kb feature-containing or central region of each of 473 TADs spanning from Chr1 to Ch19 targeted by ATTO 565-labelled imaging oligos

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