ExperimentMic multiplexed FISH on IMR-90

multiplexed FISH4DNEX3XDHENR
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Chromatin tracing in Chromosome Xi trace ID 42-81, Biological replicate 3, Technical replicate 1

   September 29th, 2023 at 6:04pm
Source Publication
Spatial organization of chromatin domains and compartments in single chromosomes.
Wang S, Su JH, et al., Science (New York, N.Y.) 2016

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSI9GU699
Biosample Biosource 
IMR-90 - 4DNSRXXGU3CH
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Highly Inclined Laminated Optical Sheet
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
1-20
20 TADs on ChrX #1-20 targeted by Alexa Fluor 647-labeled imaging oligos
ch01
1-20
20 TADs on ChrX #21-40 targeted by ATTO 565-labeled imaging oligos
ch02
0
40 TADs on ChrX #1-40 targeted by Alexa Fluor 647-labeled imaging oligos
ch03
-
fluorophore-labeled fiducial beads

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