ExperimentMic multiplexed FISH on F123-CASTx129 with Sox2 tags and RMCE site downstream of Sox2 SE

multiplexed FISH4DNEX3IBOBVO
released

Imaging the 7.5kb insert in a different location (downstream of the super enhancer) to test whether insulator function is dependent upon the precise postitioning of the insert relative to the Sox2 gene and super enhancer. We also colocalized the chromatin data with nascent RNA images to understand bursting behavior as a function of the allele classification.

   October 14th, 2021 at 3:32pm

Properties  


Experiment Type 
Follows SOP 
No
Biosample 
Biosample Modifications 
RMCE replacement
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None

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