ExperimentMic multiplexed FISH on PanIN cells from mouse

multiplexed FISH4DNEX3FHHEW9
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Multiplexed FISH in dtTomato+ GFP+ expressing (yellow) heterozygous mutant (Trp53 +/-) preinvasive pancreatic intraepithelial neoplasia (PanIN) cells from K-MADM-Trp53 mouse, Biological Relicate 1, Technical Replicate 1

   May 27th, 2025 at 5:41pm
Source Publication
Tracing the evolution of single-cell cancer 3D genomes: an atlas for cancer gene discovery.
Liu M, Jin S, et al., bioRxiv : the preprint server for biology 2024

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBS1LXLIMN
Biosample Biosource 
PanIN cells from mouse - 4DNSRU2574XG
Biosample Modifications 
Viral Transduction and Viral Transduction
Biosample Treatments 
None
Microscopy Technique 
None
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
0
Yellow-green fiducial beads for drift correction
ch01
0
Chromosomes targeted by DAPI
ch02
1-50
~60kb feature-containing or central region of each of 473 TADs spanning from Chr1 to Ch19 targeted by Alexa 647-labelled imaging oligos
ch03
1-50
~60kb feature-containing or central region of each of 473 TADs spanning from Chr1 to Ch19 targeted by ATTO 565-labelled imaging oligos

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