ExperimentMic Immunofluorescence on COS-7

Immunofluorescence4DNEX16TPSFD
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Validation experiment of fast 3D, two-color single-molecule switching nanoscopy on relatively thin targets with well-known tubular structures. This sample includes microtubules, a standard test target for single-molecule switching nanoscopy techniques.

   August 5th, 2019 at 3:32pm
Source Publication
An integrated platform for high-throughput nanoscopy.
Barentine AES, Lin Y, et al., Nature biotechnology 2023

Properties  

Experiment Type 
Immunofluorescence
Follows SOP 
No
Biosample 
4DNBSBFY99J8
Biosample Biosource 
COS-7 - 4DNSRAOF1PHU
Biosample Modifications 
None
Biosample Treatments 
Transient transfection of mEmerald-Sec61-C-18
Microscopy Technique 
STORM
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Path
 
ch00
SEC61B protein with N-terminus mEmerald targeted by anti-GFP (with anti-Rabbit, Alexa Fluor 647)
642nm
ch01
TUBA4A green monkey protein targeted by anti-alpha-Tubulin (with anti-Mouse, CF568)
560nm

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