ExperimentHiC in situ Hi-C on HCT116 with Crispr generated mClover and AID-tagged RAD21 with MboI

in situ Hi-C4DNEXU74D8FS
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in situ Hi-C on HCT-116 (HCT-116-RAD21-mAC), IAA treated to deplete Cohesin-Rad21 followed by 20 min withdrawal, treated with thymidine to inhibit DNA replication Replicate:GSM3139084_Vian-2018-HIC145

   November 27th, 2018 at 6:22pm
Source Publication
The Energetics and Physiological Impact of Cohesin Extrusion.
Vian L, Pekowska A, et al., Cell 2018

Properties  

Experiment Type 
in situ Hi-C
Follows SOP 
No
Biosample 
4DNBSACSL7KD
Digestion Enzyme 
MboI
Biosample Modifications 
None
Biosample Treatments 
IAA treatment (500.0 μM, 6.0h) and IAA washout (20.0m) and thymidine treatment (2.0 mM, 18.0h)
Biosample Biosource 
HCT116 with Crispr generated mClover and AID-tagged RAD21 - 4DNSRG1Q4WCR

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