ExperimentHiC in situ Hi-C on B cell derived cell line with MboI

in situ Hi-C4DNEXFPMBMV8
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in situ Hi-C on 30 hour activated B cells, treated with hydroxyurea to inhibit DNA replication Replicate:GSM3146474_Vian-2018-HIC020

   November 27th, 2018 at 6:20pm
Source Publication
The Energetics and Physiological Impact of Cohesin Extrusion.
Vian L, Pekowska A, et al., Cell 2018

Properties  

Experiment Type 
in situ Hi-C
Follows SOP 
No
Biosample 
4DNBSGEICEQX
Digestion Enzyme 
MboI
Biosample Modifications 
None
Biosample Treatments 
LPS (Sigma) treatment (50.0 μg/mL, 30.0h) and IL-4 (Sigma) treatment (2.5 ng/mL, 30.0h) and anti-CD180 (RP105) antibody, BD PharMingen treatment (0.5 μg/mL, 30.0h) and hydroxyurea treatment (10.0 mM, 4.0h)
Biosample Biosource 
B cell derived cell line - 4DNSRA3JY5KY

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