ExperimentHiC in situ Hi-C on HCT116 with Crispr generated mClover and AID-tagged RAD21 with MboI

in situ Hi-C4DNEXADDR8DW
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in situ Hi-C on HCT-116 (HCT-116-RAD21-mAC), IAA treated to deplete Cohesin-Rad21, treated with flavopiridol (6 hour) to inhibit transcription Replicate:GSM3139136_Vian-2018-HIC197

   November 27th, 2018 at 6:24pm
Source Publication
The Energetics and Physiological Impact of Cohesin Extrusion.
Vian L, Pekowska A, et al., Cell 2018

Properties  

Experiment Type 
in situ Hi-C
Follows SOP 
No
Biosample 
4DNBS8EQ194H
Digestion Enzyme 
MboI
Biosample Modifications 
None
Biosample Treatments 
IAA treatment (500.0 μM, 6.0h) and flavopiridol treatment (300.0 nM, 6.0h)
Biosample Biosource 
HCT116 with Crispr generated mClover and AID-tagged RAD21 - 4DNSRG1Q4WCR

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