ExperimentHiC in situ Hi-C on HCT116 with Crispr generated mClover and AID-tagged RAD21 with MboI

in situ Hi-C4DNEX5JRJMBH
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in situ Hi-C on HCT-116 (HCT-116-RAD21-mAC), IAA treated to deplete Cohesin-Rad21, treated with oligomycin (2 hour) to deplete ATP Replicate:GSM3139098_Vian-2018-HIC159

   November 27th, 2018 at 6:24pm
Source Publication
The Energetics and Physiological Impact of Cohesin Extrusion.
Vian L, Pekowska A, et al., Cell 2018

Properties  

Experiment Type 
in situ Hi-C
Follows SOP 
No
Biosample 
4DNBSVZ5SA7E
Digestion Enzyme 
MboI
Biosample Modifications 
None
Biosample Treatments 
IAA treatment (500.0 μM, 6.0h) and oligomycin treatment (0.1 μM, 2.0h) and 2-Deoxy-D-glucose treatment (5.0 mM, 2.0h)
Biosample Biosource 
HCT116 with Crispr generated mClover and AID-tagged RAD21 - 4DNSRG1Q4WCR

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