Condition test for H3K27ac PLAC-seq on GM12878 cells, biological replicate 1, condition 2: 0.3% SDS in 1xNebuffer2, 42ºC, 10 min (Step 12 in protocol) and Covaris nuclei prep (Covaris truChIP™ Chromatin Shearing Kit, section B. Nuclei Preparation, step 1-10) (Step 30 in protocol)