released
October 7th, 2019 at 5:09pm
Reference Publication
Li G et al. (2019) PMID:31384045
Li G, Liu Y, et al., Nature methods 2019
Overview
Experiment Category
Sequencing
Assay Classification
3C via Ligation
Experimental Purpose
DNA-DNA Pairwise Interactions - Single Cell
Raw Files Available
Reads (fastq) provided by lab
Assay Description
Single cell Methyl Hi-C is a method to assay chromatin
conformation and DNA methylation in single cell. The protocol
for in bulk Methyl Hi-C is followed through the steps of
proximal ligation. After ligation, nuclei are isolated by FACS
sorting and bisulfite conversion is performed on single nuclei.
Fragments are amplified and indexed to allow identification
of reads obtained from a single nucleus. Amplified libraries
are pooled and sized selected prior to sequencing. The
methylation status of cytosines and the pair-wise contact
frequencies are then determined using custom software.