ExperimentSetReplicate

ExperimentSet4DNESYXWQD8L
released
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Chromatin tracing (ORCA) of HoxA locus in TC1 mouse ESCs, with Pc factors EED and RING1b endogenously tagged with the dTAG inducible degrons, treated with dTAG-13 for 8 hours

   January 24th, 2023 at 12:49pm
Source Publication
Polycomb repression of Hox genes involves spatial feedback but not domain compaction or phase transition.
Murphy SE, Boettiger AN, Nature genetics 2024

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
stem cells
Sample 
TC1 with EED-dTAG, dTAG-Ring1b
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
Chemical
Assay Details 
Target: 52 consecutive 5-kb regions at HoxA locus (mouse)
Channel
Imaging Rounds
Path
 
ch00
1-52
52 consecutive 5-kb regions at HoxA locus (mouse) targeted by oligo probe library, readout oligo library (with Cy5-labeled imaging oligos)
ch01
1-52
52 consecutive 5-kb regions at HoxA locus (mouse) targeted by oligo probe library (with Cy3-labeled fiducial oligo probes)

4 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEX6OO8EKV
Bio Rep 1, Tec Rep 1
multiplexed FISH on TC1 with EED-dTAG, dTAG-Ring1b
File
4DNFI8OK86NF
FOF-CT - DNA-spot/trace core (csv)
1.98 MB
File
4DNFI9B5OLHF
FOF-CT - spot quality (csv)
3.18 MB
Experiment
4DNEX6COY1F2
Bio Rep 2, Tec Rep 1
multiplexed FISH on TC1 with EED-dTAG, dTAG-Ring1b
File
4DNFIYZDTHMG
FOF-CT - DNA-spot/trace core (csv)
1.95 MB
File
4DNFI8943SZ8
FOF-CT - spot quality (csv)
3.48 MB