ExperimentSetReplicate

ExperimentSet4DNESY7MOKC6
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Multiplexed FISH in HEK293T reporter cell line treated with doxycyline for 5 days causing reporter gene silencing followed by doxycycline wash-out for 22 days where reporter gene expression remains irreverisbly silenced, originally caused by 5-day recruitment of wt-KRAB (control and experiment relationships are described in detailed in 4DNFIWQ9QBOB supplementary file).

   February 4th, 2025 at 9:45pm
Source Publication
Single-cell chromatin state transitions during epigenetic memory formation.
Fujimori T, Rios-Martinez C, et al., bioRxiv : the preprint server for biology 2023

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
293T
Experiment Type(s) 
multiplexed FISH
Modification Type 
Viral Transduction
Treatment Type 
Chemical
Assay Details 
Target: Chromosomes, Custom genome assembly (pJT039-chr19 insert)
Channel
Imaging Rounds
Path
 
ch00
-
DAPI staining
ch01
1-19
Custom genome assembly (mCitrine reporter + 9 TetO sites inserted into AAVS1 safe harbor site 55115766 on chr19) targeted by Cy5-labeled imaging oligos
ch02
1-19
Custom genome assembly (mCitrine reporter + 9 TetO sites inserted into AAVS1 safe harbor site 55115766 on chr19) targeted by Cy3-labeled fiducial oligos

6 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEX9EK9V5D
Bio Rep 1, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIOAJSVA5
FOF-CT - DNA-spot/trace core (csv)
418.94 kB
Experiment
4DNEX6Z6SC5S
Bio Rep 2, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIR9AAUEM
FOF-CT - DNA-spot/trace core (csv)
345.63 kB
Experiment
4DNEXALAGI1B
Bio Rep 3, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIR9UR7AU
FOF-CT - DNA-spot/trace core (csv)
320.24 kB
Experiment
4DNEXVHG8YPH
Bio Rep 4, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIFO6FLJ7
FOF-CT - DNA-spot/trace core (csv)
707.62 kB
Experiment
4DNEX5K1FSMH
Bio Rep 5, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIXVOWBPU
FOF-CT - DNA-spot/trace core (csv)
308.56 kB
Experiment
4DNEX832BV63
Bio Rep 6, Tec Rep 1
multiplexed FISH on 293T
File
4DNFI55SPF4U
FOF-CT - DNA-spot/trace core (csv)
972.43 kB