ExperimentSetReplicate

ExperimentSet4DNESWM4U8RX
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Optical Reconstruction of Chromatin Architecture (ORCA) of a 700-kb region including the bithorax complex (BX-C) and flanking domains at 10-kb steps in fly embryos that are wild-type at the Fub locus (+/+). Additionally, 29 mRNA and nascent RNA species are detected by sequential RNA FISH.

   March 14th, 2023 at 5:37pm
Source Publication
Visualizing DNA folding and RNA in embryos at single-cell resolution.
Mateo LJ, Murphy SE, et al., Nature 2019

Microscope configuration - Mateo et al. 2019  

The experiments associated with this study are derived from either 84d52890-7e46-4cf8-810c-071ed0673ce8 or a2b6ca4e-7de9-484f-b8c3-c868b68f929e microscopes.

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
D. melanogaster
Sample Type 
tissue
Sample 
embryo
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: 29 mRNAs and nascent RNAs (fruit fly), 70 consecutive 10-kb loci in the BX-C region (fruit fly)
Channel
Path
 
ch00
70 consecutive 10-kb loci in the BX-C region (fruit fly) targeted by oligo probe library (with Cy3-labeled fiducial oligo probes)
ch01
70 consecutive 10-kb loci in the BX-C region (fruit fly) targeted by oligo probe library, readout oligo library (with Cy5-labeled imaging oligos)
ch01
29 mRNAs and nascent RNAs (fruit fly) targeted by oligo probe library, readout oligo library (with Cy5-labeled imaging oligos)

3 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEXJL6E1NS
Bio Rep 1, Tec Rep 1
multiplexed FISH on embryo
File
4DNFI5WLD5KM
FOF-CT - DNA-spot/trace core (csv)
17.98 MB
File
4DNFIWI1ZTJ9
FOF-CT - spot quality (csv)
2.58 MB
File
4DNFI7TDZY6F
FOF-CT - cell data (csv)
1.4 MB