ExperimentSetReplicate

ExperimentSet4DNESW6MXLZO
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Multiplexed FISH in HEK293T reporter cell line treated with doxycyline for 5 days causing reporter gene silencing followed by doxycycline wash-out for 22 days causing reactivation of silenced reporter gene expression, originally caused by 5-day recruitment of wt-KRAB (control and experiment relationships are described in detailed in 4DNFIWQ9QBOB supplementary file).

   February 4th, 2025 at 9:45pm
Source Publication
Single-cell chromatin state transitions during epigenetic memory formation.
Fujimori T, Rios-Martinez C, et al., bioRxiv : the preprint server for biology 2023

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
293T
Experiment Type(s) 
multiplexed FISH
Modification Type 
Viral Transduction
Treatment Type 
Chemical
Assay Details 
Target: Chromosomes, Custom genome assembly (pJT039-chr19 insert)
Channel
Imaging Rounds
Path
 
ch00
-
DAPI staining
ch01
1-19
Custom genome assembly (mCitrine reporter + 9 TetO sites inserted into AAVS1 safe harbor site 55115766 on chr19) targeted by Cy5-labeled imaging oligos
ch02
1-19
Custom genome assembly (mCitrine reporter + 9 TetO sites inserted into AAVS1 safe harbor site 55115766 on chr19) targeted by Cy3-labeled fiducial oligos

4 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEXKX2M96J
Bio Rep 1, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIEH51PI6
FOF-CT - DNA-spot/trace core (csv)
530.53 kB
Experiment
4DNEX1M4AOSH
Bio Rep 2, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIFZ4J6E2
FOF-CT - DNA-spot/trace core (csv)
556.83 kB
Experiment
4DNEXPHJ2KNK
Bio Rep 3, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIDZFP4YT
FOF-CT - DNA-spot/trace core (csv)
394.87 kB
Experiment
4DNEX4IQ9KTQ
Bio Rep 4, Tec Rep 1
multiplexed FISH on 293T
File
4DNFI1KPDOWY
FOF-CT - DNA-spot/trace core (csv)
722.7 kB