ExperimentSetReplicate

ExperimentSet4DNESUSNJ46D
released
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Validation experiment of fast 3D, two-color single-molecule switching nanoscopy on relatively thin targets with well-known tubular structures. This sample includes microtubules, a standard test target for single-molecule switching nanoscopy techniques.

   August 5th, 2019 at 3:32pm
Source Publication
An integrated platform for high-throughput nanoscopy.
Barentine AES, Lin Y, et al., Nature biotechnology 2023

Note on Localization h5 Processed Files  

Experiment Set Properties  

Set Type 
Replicate
Organism 
C. sabaeus
Sample Type 
immortalized cells
Sample 
COS-7
Experiment Type(s) 
Immunofluorescence
Modification Type 
None
Treatment Type 
Transient Transfection
Assay Details 
Target: SEC61B protein with N-terminus mEmerald, TUBA4A green monkey protein
Sample Image 
Sample frame of rendered image - Sec61B (red) and alpha-Tubulin (green)
View File Item - 4DNFIWFJH8NC
Channel
Path
 
ch00
SEC61B protein with N-terminus mEmerald targeted by anti-GFP (with anti-Rabbit, Alexa Fluor 647)
ch01
TUBA4A green monkey protein targeted by anti-alpha-Tubulin (with anti-Mouse, CF568)

1 Processed File

Experiment
File
File Type
File Size
Notes
Experiment
4DNEX16TPSFD
Bio Rep 1, Tec Rep 1
Immunofluorescence on COS-7
File
4DNFIENMMZGN
localizations (hdf5)
16.12 MB