ExperimentSetReplicate

ExperimentSet4DNESTJZUP4P
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Chromatin tracing (MINA) of an 840-kb region of chrX (chrX:77657854-78497854, GRCh38) in human female hTERT- RPE-1 cells. The data presented here is pooled from 4 biological replicates.

   August 3rd, 2022 at 10:59am
Source Publication
TAD-like single-cell domain structures exist on both active and inactive X chromosomes and persist under epigenetic perturbations.
Cheng Y, Liu M, et al., Genome biology 2021

Note on replicates  

Data from multiple experimental replicates were pooled and are presented as a single Processed File associated with this Replicate Set.

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
RPE-hTERT (Tier 2)
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: 28 consecutive 30-kb loci on chrX, mH2A.1
Channel
Imaging Rounds
Path
 
ch03
-
28 consecutive 30-kb loci on chrX targeted by Alexa Fluor 647-labeled oligo probe library
ch02
-
mH2A.1 targeted by anti-mH2A1 (with anti-Rabbit, Alexa Fluor 568)
ch03
1-14
28 consecutive 30-kb loci on chrX targeted by oligo probe library (with Alexa Fluor 647-labeled imaging oligos)
ch02
1-14
28 consecutive 30-kb loci on chrX targeted by oligo probe library (with ATTO 565-labeled imaging oligos)
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2 Processed Files

Experiment
File
File Type
File Size
From Multiple Experiments
4DNESTJZUP4P
Multiple Experiments
File
4DNFILWY147X
FOF-CT - DNA-spot/trace core (csv)
601.27 kB
File
4DNFI1ITB2WG
FOF-CT - trace data (csv)
2.55 kB
Experiment
File
File Type
File Size
Experiment
4DNEX4BFFELG
Bio Rep 1, Tec Rep 1
multiplexed FISH on RPE-hTERT (Tier 2)
-
-
-