ExperimentSetReplicate

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ExperimentSet4DNESTEL2MX4
released
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Multiplexed FISH of chromosome 22 in A549-Cas9 cancer cells from 2 coverslips where cells with PCBP1 gene knocked down using PCBP1 targeting sgRNA.

   March 28th, 2025 at 1:38pm
Source Publication
Perturb-tracing enables high-content screening of multiscale 3D genome regulators.
Cheng Y, Hu M, et al., bioRxiv : the preprint server for biology 2023

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
A549
Experiment Type(s) 
multiplexed FISH
Modification Type 
Viral Transduction
Treatment Type 
None
Assay Details 
Target: 27 TADs in Chr22, Chromosomes, Geminin
Channel
Imaging Rounds
Path
 
ch00
0
Chromosomes targeted by DAPI
ch01
1-14
Yellow-green fiducial beads for drift correction on chromosome 22
ch02
0
G1 phase detection
ch03
1-14
27 TADs imaged on Chr22 imaged using ATTO 565 imaging probes
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1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEXXIR42JU
Bio Rep 1, Tec Rep 1
multiplexed FISH on A549
File
4DNFIZUNMILA
FOF-CT - DNA-spot/trace core (csv)
8.84 MB