ExperimentSetReplicate

ExperimentSet4DNESSXNNJUB
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Chromatin tracing of a 2-Mb region on chromosome 21 (Chr21:28Mb-30Mb) in IMR90 cells across G1, S and G2 stages of the cell cycle

   September 2nd, 2022 at 10:49am
Source Publication
Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells.
Bintu B, Mateo LJ, et al., Science (New York, N.Y.) 2018

Microscope configuration - Bintu et al 2018  

The experiments associated with this study are derived from either c8e8a284-c6d0-4b97-9670-822ae7a8296f or a2b6ca4e-7de9-484f-b8c3-c868b68f929e microscopes

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
primary cells
Sample 
IMR-90
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: 65 consecutive 30-kb loci on chr21, Geminin, Chromosomes
Channel
Imaging Rounds
Path
 
ch03
1-41
65 consecutive 30-kb loci on chr21 targeted by oligo probe library (with Alexa Fluor 647-labeled readout oligos)
ch03
42-65
65 consecutive 30-kb loci on chr21 targeted by oligo probe library, readout oligo library (with Alexa Fluor 647-labeled imaging oligos)
ch01
0
Geminin targeted by anti-geminin (with anti-Rabbit, Alexa Fluor 488)
ch00
-
Chromosomes targeted by DAPI
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2 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEXXQVNRK6
Bio Rep 1, Tec Rep 1
multiplexed FISH on IMR-90
File
4DNFIORVNYGB
FOF-CT - DNA-spot/trace core (csv)
5.04 MB
File
4DNFI9I9Z8FP
FOF-CT - trace data (csv)
13.32 kB