ExperimentSetReplicate

ExperimentSet4DNESSKEUB9C
released
View JSON

Multiplexed FISH in R1 mESC of the Prdm8-Fgf5 locus using CARGO-VPR system where CARGO arrays are used to recruit dCas9-VPR at site 7.5kb upstream of the Prdm8 promoter

   September 20th, 2024 at 3:24pm
Source Publication
Long range regulation of transcription scales with genomic distance in a gene specific manner.
Jensen CL, Chen LF, et al., bioRxiv : the preprint server for biology 2024

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
stem cells
Sample 
R1 mESC line
Experiment Type(s) 
multiplexed FISH
Modification Type 
Stable Transfection
Treatment Type 
None
Assay Details 
Target: 40 5kb-regions with 5kb intervals at Prdm8-Fgf5 locus (mouse)
Channel
Path
 
ch00
40 5kb-regions with 5kb intervals at Prdm8-Fgf5 locus (mouse) targeted by oligo probe library, readout oligo library (with Cy5-labeled imaging oligos)
ch01
40 5kb-regions with 5kb intervals at Prdm8-Fgf5 locus (mouse) targeted by oligo probe library (with Cy3-labeled fiducial oligo probes) for image registration and drift correction

1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEXX8WH5HH
Bio Rep 1, Tec Rep 1
multiplexed FISH on R1 mESC line
File
4DNFIMCIZBOQ
FOF-CT - DNA-spot/trace core (csv)
7.61 MB