ExperimentSetReplicate

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ExperimentSet4DNESQJS4P53
released
View JSON

Synthetic condensation assay on tudor domain of SMN (E134K mutant)

   July 16th, 2020 at 6:24pm
Source Publication
DMA-tudor interaction modules control the specificity of in vivo condensates.
Courchaine EM, Barentine AES, et al., Cell 2021

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
immortalized cells
Sample 
NIH3T3 expressing SMN tudor (E134K) Cry2
Experiment Type(s) 
OptoDroplet
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: SMN1 engineered reagent protein with E134K Amino acid substitution, C-terminus mCherry, Cry2
Sample Image 
Sample image of NIH-3T3 cells expressing the designated Cry2 construct, 80 s after Cry2 activation
View File Item - 4DNFINGWWIAA
Channel
Path
 
ch00
SMN1 engineered reagent protein with E134K Amino acid substitution, C-terminus mCherry, Cry2

94 Processed Files

Experiment
File
File Type
File Size
Notes
From Multiple Experiments
4DNESQJS4P53
Multiple Experiments
File
4DNFI5DJIORQ
clustering metrics (zip)
57.05 kB
-
Experiment
4DNEX23RBDCE
Bio Rep 1, Tec Rep 1
OptoDroplet on NIH3T3 expressing SMN tudor (E134K) Cry2
File
4DNFIF74R2I7
image mask-nucleus (tiff)
256.26 kB
File
4DNFIJIK3ZZO
image mask-cytoplasm (tiff)
256.26 kB
File
4DNFIOP7U7KZ
image mask-cell (tiff)
256.26 kB
File
4DNFIJPETG7A
image mask-nucleus (tiff)
256.26 kB
File
4DNFIF5UQ8P4
image mask-cytoplasm (tiff)
256.26 kB
File
4DNFIBX68BX3
image mask-cell (tiff)
256.26 kB
File
4DNFICXQ8GZT
image mask-nucleus (tiff)
256.26 kB
Show 26 More Processed Files
Experiment
4DNEXQ8HEIM4
Bio Rep 2, Tec Rep 1
OptoDroplet on NIH3T3 expressing SMN tudor (E134K) Cry2
File
4DNFIK8APE6L
image mask-nucleus (tiff)
256.26 kB
File
4DNFIY6E1O1R
image mask-cytoplasm (tiff)
256.26 kB
File
4DNFI2ZSL1TR
image mask-cell (tiff)
256.26 kB
File
4DNFI6DLXLP2
image mask-nucleus (tiff)
256.26 kB
File
4DNFIBYRBS8D
image mask-cytoplasm (tiff)
256.26 kB
File
4DNFIUEQ3RBM
image mask-cell (tiff)
256.26 kB
File
4DNFIPPQL7GA
image mask-nucleus (tiff)
256.26 kB
Show 23 More Processed Files
Experiment
4DNEXRP48H7W
Bio Rep 3, Tec Rep 1
OptoDroplet on NIH3T3 expressing SMN tudor (E134K) Cry2
File
4DNFIXT3F2T6
image mask-nucleus (tiff)
256.26 kB
File
4DNFIFF3BR8D
image mask-cytoplasm (tiff)
256.26 kB
File
4DNFIX6YNTDN
image mask-cell (tiff)
256.26 kB
File
4DNFIINCEHS2
image mask-nucleus (tiff)
256.26 kB
File
4DNFI5S9NKGN
image mask-cytoplasm (tiff)
256.26 kB
File
4DNFIR4AEJLF
image mask-cell (tiff)
256.26 kB
File
4DNFI7ON4DM9
image mask-nucleus (tiff)
256.26 kB
Show 23 More Processed Files