ExperimentSetReplicate

ExperimentSet4DNESLWLKPV5
released
View JSON

Multiplexed FISH in undifferentiated mouse embryonic stem cell at NANOG locus, used as control (control and experiment relationships are described in detailed in 4DNFIWQ9QBOB supplementary file).

   February 4th, 2025 at 9:45pm
Source Publication
Single-cell chromatin state transitions during epigenetic memory formation.
Fujimori T, Rios-Martinez C, et al., bioRxiv : the preprint server for biology 2023

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
immortalized cells
Sample 
mouse embryonic stem cell
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: Chromosomes, Nanog mouse region
Channel
Imaging Rounds
Path
 
ch00
-
DAPI staining
ch01
1-19
~150-kb region around Nanog promoter targeted by by Cy5-labeled imaging oligos
ch02
1-19
~150-kb region around Nanog promoter targeted by Cy3-labeled fiducial oligos.

2 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEX7TG2QNO
Bio Rep 1, Tec Rep 1
multiplexed FISH on mouse embryonic stem cell
File
4DNFIQEZM42I
FOF-CT - DNA-spot/trace core (csv)
3.83 MB
Experiment
4DNEX38D6JNN
Bio Rep 2, Tec Rep 1
multiplexed FISH on mouse embryonic stem cell
File
4DNFIF9WYQIA
FOF-CT - DNA-spot/trace core (csv)
3.46 MB