ExperimentSetReplicate

ExperimentSet4DNESKTTMWMD
released
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Chromatin tracing (ORCA) of HoxA locus in TC1 mouse ESCs, with Pc factors EED and RING1b endogenously tagged with the dTAG inducible degrons, untreated control, cultured in 2i media

   January 24th, 2023 at 12:49pm
Source Publication
Polycomb repression of Hox genes involves spatial feedback but not domain compaction or phase transition.
Murphy SE, Boettiger AN, Nature genetics 2024

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
stem cells
Sample 
TC1 with EED-dTAG, dTAG-Ring1b
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: 52 consecutive 5-kb regions at HoxA locus (mouse)
Channel
Imaging Rounds
Path
 
ch00
1-52
52 consecutive 5-kb regions at HoxA locus (mouse) targeted by oligo probe library, readout oligo library (with Alexa Fluor 750-labeled imaging oligos)
ch01
1-52
52 consecutive 5-kb regions at HoxA locus (mouse) targeted by oligo probe library (with Cy3-labeled fiducial oligo probes)

4 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEXNZ5ZZF8
Bio Rep 1, Tec Rep 1
multiplexed FISH on TC1 with EED-dTAG, dTAG-Ring1b
File
4DNFI9J3L3G9
FOF-CT - DNA-spot/trace core (csv)
5.75 MB
File
4DNFIS36Z4W8
FOF-CT - spot quality (csv)
8.45 MB
Experiment
4DNEXQOYR427
Bio Rep 2, Tec Rep 1
multiplexed FISH on TC1 with EED-dTAG, dTAG-Ring1b
File
4DNFIKVDFH44
FOF-CT - DNA-spot/trace core (csv)
2.96 MB
File
4DNFILNK6AIR
FOF-CT - spot quality (csv)
5.18 MB