ExperimentSetReplicate

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ExperimentSet4DNESHA7AS6L
released
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Multiplexed FISH of chromosome 22 in neural crest cells from 2 coverslips where cells, differentiated from hESCs transfected with CHD7 targeting shRNA causing CHD7 knocked-down

   March 28th, 2025 at 1:38pm
Source Publication
Perturb-tracing enables high-content screening of multiscale 3D genome regulators.
Cheng Y, Hu M, et al., bioRxiv : the preprint server for biology 2023

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
in vitro differentiated cells
Sample 
H1-hESC differentiated to neural progenitor cell
Experiment Type(s) 
multiplexed FISH
Modification Type 
Viral Transduction
Treatment Type 
None
Assay Details 
Target: 27 TADs in Chr22, Chromosomes, Geminin
Channel
Imaging Rounds
Path
 
ch00
0
Chromosomes targeted by DAPI
ch01
1-14
Yellow-green fiducial beads for drift correction on chromosome 22
ch02
0
G1 phase detection
ch03
1-14
27 TADs imaged on Chr22 imaged using ATTO 565 imaging probes
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1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEXZ1WOI1D
Bio Rep 1, Tec Rep 1
multiplexed FISH on H1-hESC differentiated to neural progenitor cell
File
4DNFIRX951K7
FOF-CT - DNA-spot/trace core (csv)
11.27 MB