ExperimentSetReplicate

ExperimentSet4DNESGAO6AWG
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Chromatin tracing of a 2-Mb region on chromosome 21 (Chr21:28Mb-30Mb) in HCT116 cells with RAD21-AID, treated with auxin

   September 2nd, 2022 at 10:49am
Source Publication
Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells.
Bintu B, Mateo LJ, et al., Science (New York, N.Y.) 2018

Microscope configuration - Bintu et al 2018  

The experiments associated with this study are derived from either c8e8a284-c6d0-4b97-9670-822ae7a8296f or a2b6ca4e-7de9-484f-b8c3-c868b68f929e microscopes

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
HCT116 with Crispr generated mClover and AID-tagged RAD21
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
Chemical
Assay Details 
Target: 65 consecutive 30-kb loci on chr21
Channel
Imaging Rounds
Path
 
ch03
1-41
65 consecutive 30-kb loci on chr21 targeted by oligo probe library (with Alexa Fluor 647-labeled readout oligos)
ch03
42-65
65 consecutive 30-kb loci on chr21 targeted by oligo probe library, readout oligo library (with Alexa Fluor 647-labeled imaging oligos)
ch02
-
540/560nm fiducial beads

1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEXKOP7LYG
Bio Rep 1, Tec Rep 1
multiplexed FISH on HCT116 with Crispr generated mClover and AID-tagged RAD21
File
4DNFIZBE2Z7G
FOF-CT - DNA-spot/trace core (csv)
22.77 MB