ExperimentSetReplicate

ExperimentSet4DNESCCHHMAL
released
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Multiplexed FISH in HEK293T reporter cell line treated with doxycyline for 1 day, causing recruitment of KRAB mutant (Y46A) which is able to silence reporter gene activation but with reduced epigenetic memory compared to wt-KRAB (control and experiment relationships are described in detailed in 4DNFIWQ9QBOB supplementary file).

   February 4th, 2025 at 9:45pm
Source Publication
Single-cell chromatin state transitions during epigenetic memory formation.
Fujimori T, Rios-Martinez C, et al., bioRxiv : the preprint server for biology 2023

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
293T
Experiment Type(s) 
multiplexed FISH
Modification Type 
Viral Transduction
Treatment Type 
Chemical
Assay Details 
Target: Chromosomes, Custom genome assembly (pJT039-chr19 insert)
Channel
Imaging Rounds
Path
 
ch00
-
DAPI staining
ch01
1-19
Custom genome assembly (mCitrine reporter + 9 TetO sites inserted into AAVS1 safe harbor site 55115766 on chr19) targeted by Cy5-labeled imaging oligos
ch02
1-19
Custom genome assembly (mCitrine reporter + 9 TetO sites inserted into AAVS1 safe harbor site 55115766 on chr19) targeted by Cy3-labeled fiducial oligos

2 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEXE26M4XW
Bio Rep 1, Tec Rep 1
multiplexed FISH on 293T
File
4DNFI7MS783R
FOF-CT - DNA-spot/trace core (csv)
826.7 kB
Experiment
4DNEXDNMXR3R
Bio Rep 2, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIQMNMO3T
FOF-CT - DNA-spot/trace core (csv)
585.59 kB