ExperimentSetReplicate

Browser Suggestion

Google Chrome  or Mozilla Firefox are the recommended browser(s) for using the 4DN Data Portal.

Microsoft Edge, Safari, Opera etc. should work for a majority of portal functions but are not explicitly supported and may present some glitches, e.g. during submission.

ExperimentSet4DNESB7SZY5Q
released
View JSON

Multiplexed FISH of chromosome 22 in neural crest cells from 3 coverslips where cells differentiated from hESCs transfected with non targeting shRNA used as control for shCHD7

   March 28th, 2025 at 1:38pm
Source Publication
Perturb-tracing enables high-content screening of multiscale 3D genome regulators.
Cheng Y, Hu M, et al., bioRxiv : the preprint server for biology 2023

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
in vitro differentiated cells
Sample 
H1-hESC differentiated to neural progenitor cell
Experiment Type(s) 
multiplexed FISH
Modification Type 
Viral Transduction
Treatment Type 
None
Assay Details 
Target: 27 TADs in Chr22, Chromosomes, Geminin
Channel
Imaging Rounds
Path
 
ch00
0
Chromosomes targeted by DAPI
ch01
1-14
Yellow-green fiducial beads for drift correction on chromosome 22
ch02
0
G1 phase detection
ch03
1-14
27 TADs imaged on Chr22 imaged using ATTO 565 imaging probes
Show 1 More

1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEXMZ481XR
Bio Rep 1, Tec Rep 1
multiplexed FISH on H1-hESC differentiated to neural progenitor cell
File
4DNFIOXTQT5K
FOF-CT - DNA-spot/trace core (csv)
6.89 MB