ExperimentSetReplicate

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ExperimentSet4DNESAN7TNP6
released
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Confocal microscopy experiments to compare the detection of TElomeric Repeat-containing RNA (TERRA) by RNA FISH and by dCas13-Suntag-sfGFP in U2OS cells

   November 4th, 2021 at 4:06pm
Source Publication
CRISPR Cas13-Based Tools to Track and Manipulate Endogenous Telomeric Repeat-Containing RNAs in Live Cells.
Xu M, Chigumira T, et al., Frontiers in molecular biosciences 2021

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
U2OS
Experiment Type(s) 
RNA FISH
Modification Type 
None
Treatment Type 
Transient Transfection
Assay Details 
Target: Chromosomes, TERRA
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
TERRA targeted by TERRA gRNA, dCas13b-SunTag (with sfGFP-labeled scFv)
ch02
TERRA targeted by Cy3-labeled custom DNA oligos

3 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEX8JTLUFF
Bio Rep 1, Tec Rep 1
RNA FISH on U2OS
File
4DNFIZ2RQ9HK
tsv
683 Bytes
Experiment
4DNEX6XJC3ZV
Bio Rep 2, Tec Rep 1
RNA FISH on U2OS
File
4DNFIU699OTS
tsv
737 Bytes
Experiment
4DNEX6BLN69C
Bio Rep 3, Tec Rep 1
RNA FISH on U2OS
File
4DNFISESK1YG
tsv
812 Bytes