ExperimentSetReplicate

ExperimentSet4DNESAF7ROIW
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Multiplexed FISH (Hi-M) of the dpp locus in intact drosophila embryos at nuclear cycle 14 (nc14)

   September 10th, 2024 at 2:52pm
Source Publication
3D chromatin interactions involving Drosophila insulators are infrequent but preferential and arise before TADs and transcription.
Messina O, Raynal F, et al., Nature communications 2023

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
D. melanogaster
Sample Type 
whole organisms
Sample 
whole fruit fly
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: 34 oligos in dpp locus (fruit fly), Chromosomes
Channel
Imaging Rounds
Path
 
ch00
0
Nuclei labelled by DAPI (first acquisition)
ch01
0
Fiducial mark acquired using Atto 550 (first acquisition)
ch02
0
Hybridization of imaging oligo to the reverse primer of the oligopaint library labelled with alexa flour 647 (first acquisition: mask0)
ch00
1-34
34 sequential ~12-kb loci in the dpp loci (fruit fly) targeted by oligo probe library (with atto-550-labelled fiducial oligo probes)
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1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEXEEZL7FB
Bio Rep 1, Tec Rep 1
multiplexed FISH on whole fruit fly
File
4DNFI1T9J25Q
FOF-CT - DNA-spot/trace core (csv)
15.7 MB