ExperimentSetReplicate

ExperimentSet4DNESA84SNKC
released
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DNA-FISH with probe pPK1003

   December 2nd, 2019 at 8:40am
Source Publication
Distinct features of nucleolus-associated domains in mouse embryonic stem cells.
Bizhanova A, Yan A, et al., Chromosoma 2020

Assay Description  

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
stem cells
Sample 
F121-9-CASTx129 (Tier 2)
Experiment Type(s) 
DNA FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: Nucleolus, GRCm38:15:84216729-84409126 mouse region, Chromosomes
Sample Image 
Sample Image - DNA FISH (green) with Fibrillarin (nucleolus, red) and DAPI (blue) co-staining
View File Item - 4DNFIOFUTRNO
Channel
Path
 
ch00
Nucleolus targeted by Rabbit Anti-Fibrillarin Antibody (with Alexa Fluor 594-labeled Anti-Rabbit Secondary Antibody)
ch01
GRCm38:15:84216729-84409126 mouse region targeted by Biotin (with Alexa Fluor 488-labeled Streptavidin)
ch02
Chromosomes targeted by DAPI

3 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEX2JFQ5QS
Bio Rep 1, Tec Rep 1
DNA FISH on F121-9-CASTx129 (Tier 2)
File
4DNFI8CED1QZ
locus distances, submitter format (txt)
572 Bytes
Experiment
4DNEXUN1NJAY
Bio Rep 2, Tec Rep 1
DNA FISH on F121-9-CASTx129 (Tier 2)
File
4DNFIULS5IMX
locus distances, submitter format (txt)
379 Bytes
Experiment
4DNEXXVBRW5E
Bio Rep 3, Tec Rep 1
DNA FISH on F121-9-CASTx129 (Tier 2)
File
4DNFITB6SNVN
locus distances, submitter format (txt)
635 Bytes