ExperimentSetReplicate

ExperimentSet4DNESA6K6PSP
released
View JSON

Multiplexed FISH in untreated HEK293T reporter cell line used as control where gene expression of reporter is active in cells (control and experiment relationships are described in detailed in 4DNFIWQ9QBOB supplementary file).

   February 4th, 2025 at 9:45pm
Source Publication
Single-cell chromatin state transitions during epigenetic memory formation.
Fujimori T, Rios-Martinez C, et al., bioRxiv : the preprint server for biology 2023

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
293T
Experiment Type(s) 
multiplexed FISH
Modification Type 
Viral Transduction
Treatment Type 
None
Assay Details 
Target: Chromosomes, Custom genome assembly (pJT039-chr19 insert)
Channel
Imaging Rounds
Path
 
ch00
-
DAPI staining
ch01
1-19
Custom genome assembly (mCitrine reporter + 9 TetO sites inserted into AAVS1 safe harbor site 55115766 on chr19) targeted by Cy5-labeled imaging oligos
ch02
1-19
Custom genome assembly (mCitrine reporter + 9 TetO sites inserted into AAVS1 safe harbor site 55115766 on chr19) targeted by Cy3-labeled fiducial oligos

8 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEX9Y1SBQM
Bio Rep 1, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIZ11DU3H
FOF-CT - DNA-spot/trace core (csv)
868.44 kB
Experiment
4DNEXHE8CD1V
Bio Rep 2, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIQ3XDHUG
FOF-CT - DNA-spot/trace core (csv)
821.63 kB
Experiment
4DNEXOR1FZEA
Bio Rep 3, Tec Rep 1
multiplexed FISH on 293T
File
4DNFI8AVWUVZ
FOF-CT - DNA-spot/trace core (csv)
411.66 kB
Experiment
4DNEXT97O4XD
Bio Rep 4, Tec Rep 1
multiplexed FISH on 293T
File
4DNFI7DT93YY
FOF-CT - DNA-spot/trace core (csv)
681.59 kB
Experiment
4DNEXMYDDIGD
Bio Rep 5, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIWMEUOAV
FOF-CT - DNA-spot/trace core (csv)
177.86 kB
Experiment
4DNEX7UEIQXF
Bio Rep 6, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIHRIIW9P
FOF-CT - DNA-spot/trace core (csv)
388.26 kB
Experiment
4DNEX75IJYLG
Bio Rep 7, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIYK7IHLX
FOF-CT - DNA-spot/trace core (csv)
494.54 kB
Experiment
4DNEXDC6TZHG
Bio Rep 8, Tec Rep 1
multiplexed FISH on 293T
File
4DNFIEF8WYBD
FOF-CT - DNA-spot/trace core (csv)
941.7 kB