ExperimentSetReplicate

ExperimentSet4DNESA5PN8AB
released

Micro-C on HeLa-S3 (non synchronized) with 1% Formaldehyde and 3mM EGS crosslinking

   June 27th, 2020 at 6:56pm
Source Publication
Systematic evaluation of chromosome conformation capture assays.
Akgol Oksuz B, Yang L, et al., Nature methods 2021

WARNING: non-optimal cross-linking conditions  


This dataset may be obtained with non-optimal cross-linking conditions (as demonstrated in the paper) and is reported here for documentation purposes.

Users interested in using Hi-C or Micro-C data from this project for alternative analyses are advised to use only datasets generated with the optimal cross-linking conditions of 1% Formaldehyde + 3mM DSG. The study authors further advise users to carefully select digestion enzymes and library size to fit their needs. A list of preferred datasets for re-use is provided.


Experiment Set Properties  


Set Type 
Replicate
Organism 
Sample Type 
immortalized cells
Sample 
Experiment Type(s) 
Modification Type 
None
Treatment Type 
None
Assay Details 
Enzyme: MNase

8 Processed Files

Quality Metrics

Experiment
For File
Filtered Reads
Cis reads (>20kb)
Short cis reads
Trans Reads
Report
Details
From Multiple Experiments
4DNESA5PN8AB
Multiple Experiments
File
Contact List-combined (pairs)
4DNFILD8JK1M
221.98m
23.9%
67.933%
8.167%
Experiment
4DNEXDGA81X4
Bio Rep 1, Tec Rep 1
Micro-C on HeLa-S3 with MNase
File
Contact List-replicate (pairs)
4DNFI16EIJYE
221.98m
23.9%
67.933%
8.167%
Experiment
For File
Failed Balancing
Details
From Multiple Experiments
4DNESA5PN8AB
Multiple Experiments
File
Contact Matrix (mcool)
4DNFIM48WBOE
None
Experiment
For File
Total Reads
Unmapped Reads
Multimapped Reads
Duplicate Reads
Walks
Minor Contigs
Details
Experiment
4DNEXDGA81X4
Bio Rep 1, Tec Rep 1
Micro-C on HeLa-S3 with MNase
File
Alignments (bam)
4DNFIR96Y51E
352.53m
4.089%
18.085%
14.852%
0.006%
0.04%