ExperimentSetReplicate

ExperimentSet4DNES9VPS41V
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DamID for Nucleolus (4X-AP3) on HFF wildtype cells - cells were transduced with virus expressing a Dam fusion protein, gDNA was harvested after 3 or 4 days and processed for DamID-seq

   July 1st, 2020 at 7:53am

Caution:  

Please use caution when using this data. Nucleolar DamID has not been validated extensively. Preliminary analyses suggest a substantial difference in data quality between cell lines. We interpret this as a difference in information content along the chromosomes due to more pronounced nucleolar interactions in some cell lines.

Data Usage Guidelines  

Data Use Guidelines: This is a data set generated by the 4DN Network and made freely available to the scientific community. If you are intending to use these data for a publication, we ask that you please contact the data generating lab to discuss possible coordinated publication. In your manuscript, please cite the 4DN White Paper (doi:10.1038/nature23884) and the 4DN Data Portal paper (doi:10.1038/s41467-022-29697-4), and please acknowledge the 4DN lab which generated the data. Please direct any questions to the Data Coordination and Integration Center.

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
HFFc6 (Tier 1)
Experiment Type(s) 
DamID-seq
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: Nucleolus Localization Sequence (4X-AP3D1) (engineered reagent)

9 Processed Files