ExperimentSetReplicate

ExperimentSet4DNES9PJO3YW
released
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Validation experiment of high-throughput 3D, two-color single-molecule switching nanoscopy. This experiment tested whether the fast speeds attained by our imaging platform were sustainable for over 24 hours, and additionally served as a test of large-scale SMS nanoscopy image analysis.

   August 5th, 2019 at 3:32pm
Source Publication
An integrated platform for high-throughput nanoscopy.
Barentine AES, Lin Y, et al., Nature biotechnology 2023

Note on Localization h5 Processed Files  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
U2OS (Tier 2)
Experiment Type(s) 
Immunofluorescence
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: NPM1 protein, LMNB1 protein
Sample Image 
Sample rendered image - Lamin B (green) and Nucleophosmin (red)
View File Item - 4DNFI5RC3LNA
Channel
Path
 
ch00
NPM1 protein targeted by anti-nucleophosmin (with anti-Mouse, Alexa Fluor 647)
ch01
LMNB1 protein targeted by anti-LaminB1 (with anti-Rabbit, CF568)

7 Processed Files

Experiment
File
File Type
File Size
Notes
Experiment
4DNEX76VDXUJ
Bio Rep 1, Tec Rep 1
Immunofluorescence on U2OS (Tier 2)
File
4DNFI99C2KTG
localizations (tar)
24.91 GB
File
4DNFI1ZDARZT
localizations (hdf5)
2.03 MB
File
4DNFIIMK538C
localizations (hdf5)
1.13 MB
File
4DNFIIY3XD8S
localizations (hdf5)
1.25 MB
File
4DNFI4RPQ4NA
localizations (hdf5)
3.54 MB
File
4DNFIUWOKI2R
localizations (hdf5)
1.63 MB
File
4DNFIZ61CABZ
localizations (hdf5)
1.57 MB