ExperimentSetReplicate

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ExperimentSet4DNES9946GB9
released
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Multiplexed FISH in Sox2 tagged mouse embryonic stem cell line (F123-CASTx129) with a 7.5kb insertion containing 4 CTCF binding sites introduced between Sox2 and its Super Enhancer, treated with dTAG to degrade CTCF and A485 to inhibit p300 activity for 12 hrs.

   February 3rd, 2025 at 3:47pm

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
stem cells
Sample 
F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE
Experiment Type(s) 
multiplexed FISH
Modification Type 
Crispr
Treatment Type 
Chemical
Assay Details 
Target: 101 40-bp regions on Chr11 (mouse), 4470 40-bp on the 205kb stretch from Sox2 gene to SE and surrounding regions (mouse), Chromosomes
Channel
Imaging Rounds
Path
 
ch00
1
DAPI staining
ch03
1
101 40-bp regions on Chr11 (mouse) placed as a 7.5kb insertion between Sox2 promoter and SE, targeted by Alexa Fluor 647 (Imaging probes)
ch01
2-15
4470 40-bp on the 205kb stretch from Sox2 gene to SE and surrounding regions (mouse) targeted by Fluospheres 505 (Fiducial probes)
ch02
2-15
4470 40-bp on the 205kb stretch from Sox2 gene to SE and surrounding regions (mouse) targeted by Alexa Fluor 750 (Imaging probes)
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1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEXR61ZVQ1
Bio Rep 1, Tec Rep 1
multiplexed FISH on F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE
File
4DNFIVP2CYLF
FOF-CT - DNA-spot/trace core (csv)
6.04 MB