ExperimentSetReplicate

ExperimentSet4DNES94LRC75
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Multiplexed FISH of chromosome 21 in RPE-1 cells, with CHD7 knocked down using CHD7 targeting siRNA

   March 28th, 2025 at 1:38pm
Source Publication
Perturb-tracing enables high-content screening of multiscale 3D genome regulators.
Cheng Y, Hu M, et al., bioRxiv : the preprint server for biology 2023

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
RPE-hTERT (Tier 2)
Experiment Type(s) 
multiplexed FISH
Modification Type 
Viral Transduction
Treatment Type 
None
Assay Details 
Target: 34 TADs in Chr21, Chromosomes, Geminin
Channel
Imaging Rounds
Path
 
ch00
0
Chromosomes targeted by DAPI
ch01
1-17
Yellow-green fiducial beads for drift correction on chromosome 21
ch02
0
G1 phase detection
ch03
1-17
34 TADs imaged on Chr21 imaged using ATTO 560 imaging probes
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1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEX9B77NC7
Bio Rep 1, Tec Rep 1
multiplexed FISH on RPE-hTERT (Tier 2)
File
4DNFI7A349T7
FOF-CT - DNA-spot/trace core (csv)
527.74 kB