ExperimentSetReplicate

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ExperimentSet4DNES8M2U3VI
released
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ATAC-seq on HEK293 cells - 4h after light Cas9-AluGG pcRNA (light-deactivatable CRISPR)

   February 14th, 2022 at 3:09am
Source Publication
Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites.
Zou RS, Marin-Gonzalez A, et al., Nature cell biology 2022

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
HEK293 (Tier 2)
Experiment Type(s) 
ATAC-seq
Modification Type 
None
Treatment Type 
  1. Transient Transfection
  2. Light
Assay Details 
None

5 Processed Files

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Experiment
File
File Type
File Size
From Multiple Experiments
4DNES8M2U3VI
Multiple Experiments
File
4DNFIWTM2NKV
peaks (bigbed)
10.17 MB
File
4DNFIGP6S6XT
conservative peaks (bigbed)
10.11 MB
File
4DNFI7RB773L
signal fold change (bw)
2.46 GB
Experiment
4DNEXKGKGBNH
Bio Rep 1, Tec Rep 1
ATAC-seq on HEK293 (Tier 2)
File
4DNFIQP4GNF5
read positions (bed)
1016.21 MB
Experiment
4DNEXSC9TIFV
Bio Rep 2, Tec Rep 1
ATAC-seq on HEK293 (Tier 2)
File
4DNFIHRQ3G9H
read positions (bed)
1.37 GB

Quality Metrics

Experiment
For File
Percent mitochondrial reads
Nonredundant Read Fraction (NRF)
PCR Bottleneck Coefficient (PBC)
Filtered & Deduped Reads
Details
Experiment
4DNEXKGKGBNH
Bio Rep 1, Tec Rep 1
ATAC-seq on HEK293 (Tier 2)
File
Read Positions (bed)
4DNFIQP4GNF5
8.85%
0.92
0.93
63.36m
Experiment
4DNEXSC9TIFV
Bio Rep 2, Tec Rep 1
ATAC-seq on HEK293 (Tier 2)
File
Read Positions (bed)
4DNFIHRQ3G9H
7.11%
0.92
0.92
87.28m
Experiment
For File
Optimal Peaks
Rescue Ratio
Self Consistency Ratio
Fraction of Reads in Peaks
Details
From Multiple Experiments
4DNES8M2U3VI
Multiple Experiments
File
Peaks (bigbed)
4DNFIWTM2NKV
223.5k
1.01
1.02
0.3