ExperimentSetReplicate

ExperimentSet4DNES7V47RFL
released
View JSON

Multiplexed FISH in GFP+ expressing (green) homozygous mutant (Trp53 -/-) PDAC cells from K-MADM-Trp53 mouse

   May 27th, 2025 at 5:42pm
Source Publication
Tracing the evolution of single-cell cancer 3D genomes: an atlas for cancer gene discovery.
Liu M, Jin S, et al., bioRxiv : the preprint server for biology 2024

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
primary cells
Sample 
PDAC cells from mouse SA8068
Experiment Type(s) 
multiplexed FISH
Modification Type 
Viral Transduction
Treatment Type 
None
Assay Details 
Target: Chromosomes, Total 473 TADs spanning between Chr1-Chr19 (mouse)
Channel
Imaging Rounds
Path
 
ch00
0
Yellow-green fiducial beads for drift correction
ch01
0
Chromosomes targeted by DAPI
ch02
1-50
~60kb feature-containing or central region of each of 473 TADs spanning from Chr1 to Ch19 targeted by Alexa 647-labelled imaging oligos
ch03
1-50
~60kb feature-containing or central region of each of 473 TADs spanning from Chr1 to Ch19 targeted by ATTO 565-labelled imaging oligos

1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEX5MDGP7T
Bio Rep 1, Tec Rep 1
multiplexed FISH on PDAC cells from mouse SA8068
File
4DNFIGQ7OKN3
FOF-CT - DNA-spot/trace core (csv)
2.15 MB