ExperimentSetReplicate

Browser Suggestion

Google Chrome  or Mozilla Firefox are the recommended browser(s) for using the 4DN Data Portal.

Microsoft Edge, Safari, Opera etc. should work for a majority of portal functions but are not explicitly supported and may present some glitches, e.g. during submission.

ExperimentSet4DNES79RMWNZ
released
View JSON

Single molecule mrnaFISH on endogenous Krüppel using dual color 5' and 3' block probe sets

   November 5th, 2019 at 9:03am
Source Publication
Diverse Spatial Expression Patterns Emerge from Unified Kinetics of Transcriptional Bursting.
Zoller B, Little SC, et al., Cell 2018

Experiment Set Properties  

Set Type 
Replicate
Organism 
D. melanogaster
Sample Type 
whole organisms
Sample 
Fruit fly (Oregon-R) embryo - NC13
Experiment Type(s) 
RNA FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: 8' region of knuppel mRNA (fruit fly), Chromosomes
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
5' region of knuppel mRNA (fruit fly) targeted by ATTO 565-labeled custom DNA oligos
ch02
3' region of knuppel mRNA (fruit fly) targeted by ATTO 633-labeled custom DNA oligos

11 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEXJQDED4A
Bio Rep 1, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFIXMS8HS2
microscopy summary (csv)
48.83 kB
Experiment
4DNEXYJLAW2H
Bio Rep 2, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFIAW9ISRZ
microscopy summary (csv)
42.79 kB
Experiment
4DNEXG34CV9E
Bio Rep 3, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFIG8LYEK3
microscopy summary (csv)
48.38 kB
Experiment
4DNEXSCV828M
Bio Rep 4, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFIT9WYT47
microscopy summary (csv)
45.26 kB
Experiment
4DNEXCFZFVO8
Bio Rep 5, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFI3N2WSW6
microscopy summary (csv)
45.49 kB
Experiment
4DNEX2W95TCL
Bio Rep 6, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFI2P96B31
microscopy summary (csv)
47.43 kB
Experiment
4DNEX5R8XBPD
Bio Rep 7, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFINX8VD9B
microscopy summary (csv)
50.12 kB
Experiment
4DNEX6ICXFIN
Bio Rep 8, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFI23W2MOM
microscopy summary (csv)
55.12 kB
Experiment
4DNEX27HQIKN
Bio Rep 9, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFINVKULKO
microscopy summary (csv)
43.74 kB
Experiment
4DNEXRJCTDA8
Bio Rep 10, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFIN7PEBXT
microscopy summary (csv)
43.16 kB
Experiment
4DNEX432GNU9
Bio Rep 11, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFIG3KXCX7
microscopy summary (csv)
41.43 kB
Show 4 More