ExperimentSetReplicate

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ExperimentSet4DNES6MUQJPB
released
View JSON

Single molecule mrnaFISH on endogenous hunchback using dual color 5' and 3' block probe sets

   November 5th, 2019 at 9:03am
Source Publication
Diverse Spatial Expression Patterns Emerge from Unified Kinetics of Transcriptional Bursting.
Zoller B, Little SC, et al., Cell 2018

Experiment Set Properties  

Set Type 
Replicate
Organism 
D. melanogaster
Sample Type 
whole organisms
Sample 
Fruit fly (Oregon-R) embryo - NC13
Experiment Type(s) 
RNA FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: 8' region of hunchback mRNA (fruit fly), Chromosomes
Sample Image 
Sample RNA FISH image - 20X fly embryo with hunchback mRNA labeled
View File Item - 4DNFIRWBGPUE
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
5' region of hunchback mRNA (fruit fly) targeted by ATTO 565-labeled custom DNA oligos
ch02
3' region of hunchback mRNA (fruit fly) targeted by ATTO 633-labeled custom DNA oligos

18 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEX4V6R1P2
Bio Rep 1, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFISSPCEZX
microscopy summary (csv)
45.9 kB
Experiment
4DNEXJIKO4FL
Bio Rep 2, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFI5DMDOTS
microscopy summary (csv)
48.16 kB
Experiment
4DNEXYLVNA2V
Bio Rep 3, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFI5T3Z7TD
microscopy summary (csv)
45.4 kB
Experiment
4DNEXZXWTKYR
Bio Rep 4, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFIQOMVW3M
microscopy summary (csv)
49.36 kB
Experiment
4DNEXP22T7J6
Bio Rep 5, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFISLGN4MI
microscopy summary (csv)
46.84 kB
Experiment
4DNEX2SU3IUJ
Bio Rep 6, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFIEOAV4O4
microscopy summary (csv)
52.08 kB
Experiment
4DNEX947YPS1
Bio Rep 7, Tec Rep 1
RNA FISH on Fruit fly (Oregon-R) embryo - NC13
File
4DNFI8H1TDP3
microscopy summary (csv)
53.7 kB
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