ExperimentSetReplicate

ExperimentSet4DNES6LAM7TG
released
View JSON

Chromatin tracing (ORCA) of HoxA locus in parental TC1 mouse ESCs, differentiated to cervical neural progenitor cells

   January 24th, 2023 at 12:49pm
Source Publication
Polycomb repression of Hox genes involves spatial feedback but not domain compaction or phase transition.
Murphy SE, Boettiger AN, Nature genetics 2024

Microscope configuration  

The experiments associated with this study are derived from either 33ea326f-a557-4457-becf-55bc860c4bdf or 4fc82c6a-a9cd-43e1-aee8-36db3174b409 microscopes

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
in vitro differentiated cells
Sample 
TC1 differentiated to neural progenitor cell
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: 52 consecutive 5-kb regions at HoxA locus (mouse)
Channel
Imaging Rounds
Path
 
ch00
1-52
52 consecutive 5-kb regions at HoxA locus (mouse) targeted by oligo probe library, readout oligo library (with Alexa Fluor 750-labeled imaging oligos)
ch01
1-52
52 consecutive 5-kb regions at HoxA locus (mouse) targeted by oligo probe library (with Cy3-labeled fiducial oligo probes)

4 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEXMQAGEQ4
Bio Rep 1, Tec Rep 1
multiplexed FISH on TC1 differentiated to neural progenitor cell
File
4DNFIKCHMBEC
FOF-CT - DNA-spot/trace core (csv)
6.3 MB
File
4DNFIZEALX3X
FOF-CT - spot quality (csv)
9.24 MB
Experiment
4DNEX5GZAWUH
Bio Rep 2, Tec Rep 1
multiplexed FISH on TC1 differentiated to neural progenitor cell
File
4DNFI9FGBEOF
FOF-CT - DNA-spot/trace core (csv)
14.99 MB
File
4DNFI1P3SRWE
FOF-CT - spot quality (csv)
25.9 MB