ExperimentSetReplicate

ExperimentSet4DNES5C3XVFP
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DNA-MERFISH combined with RNA-MERFISH to detect nascent RNA transcripts of 1137 genes overlapping with 1041 genomic loci targeted by DNA-MERFISH

   October 17th, 2024 at 2:36pm
Source Publication
Genome-Scale Imaging of the 3D Organization and Transcriptional Activity of Chromatin.
Su JH, Zheng P, et al., Cell 2020

Microscope configuration - Su JH et al. 2020  

The experiments associated with this study are derived from either b489483d-e203-45e1-b52f-fe5e8c8538e0 or 849c69ce-a0b1-4fe7-8337-ebfc7b308622 microscopes.

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
primary cells
Sample 
IMR-90
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: Chromosomes, transcript, region
Channel
Imaging Rounds
Path
 
ch00
-
Chromosomes targeted by DAPI
ch01
1-18
RNA-MERFISH to detect 1137 genes overlapping 1041 genomic loci (targeted by DNA MERFISH) by Alexa Flour 750-labeled imaging oligos
ch02
1-18
RNA-MERFISH to detect 1137 genes overlapping 1041 genomic loci (targeted by DNA MERFISH) by Alexa Flour 647-labeled imaging oligos
ch03
1-18
RNA-MERFISH to detect 1137 genes overlapping 1041 genomic loci (targeted by DNA MERFISH) by Cy3-labeled imaging oligos
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3 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEXCWE7KU7
Bio Rep 1, Tec Rep 1
multiplexed FISH on IMR-90
File
4DNFIXMERLJQ
FOF-CT - DNA-spot/trace core (csv)
492.23 MB
File
4DNFI7UYX9PP
FOF-CT - spot biological data (csv)
175.75 MB
File
4DNFI6HBZKKH
FOF-CT - RNA-spot (csv)
286.68 MB