ExperimentSetReplicate

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ExperimentSet4DNES44GBU46
released
View JSON

RNA FISH for nascent transcription of genes combined with ORCA labeling for 44 Super Ehancer (SE) regions in mouse embryonic stem cells ES-E14TG2a

   May 21st, 2024 at 4:58pm
Source Publication
Super-enhancer interactomes from single cells link clustering and transcription.
Le DJ, Hafner A, et al., bioRxiv : the preprint server for biology 2024

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
stem cells
Sample 
ES-E14TG2a
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: 374 genomic regions, 21 transcripts
Channel
Imaging Rounds
Path
 
ch00
1-188
ORCA labeling of 188 SEs imaged using Alexa 647
ch01
1-188
ORCA labeling of 188 SEs imaged using Alexa 750
ch02
-
Image registration and drift correction using Cy3
ch03
-
ORCA labeling of 44 SEs combined with RNA-FISH

3 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEXFU8K95M
Bio Rep 1, Tec Rep 1
multiplexed FISH on ES-E14TG2a
File
4DNFIJ5YBMVT
FOF-CT - cell data (csv)
6.07 MB
File
4DNFI4YJYP5Q
FOF-CT - DNA-spot/trace core (csv)
11.5 MB
File
4DNFIF8577IV
FOF-CT - spot quality (csv)
16.21 MB